Rohit Bhargava

Professor
Biomedical and Translational Sciences

rxb@illinois.edu

For More Information

Education

Ph.D. (Macromolecular Science and Engineering), Case Western Reserve University, Cleveland, Ohio, 2000.

Academic Positions

Director, Cancer Center at Illinois, University of Illinois at Urbana-Champaign, 2016-present
Professor, Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL. 2012-present
Founder Professor in Engineering, Grainger Engineering Breakthroughs Initiative, University of Illinois at Urbana-Champaign, Urbana, IL. 2016-2022
Full-time faculty, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, 2005-present
Brunner Fellow for Educational Innovation, Academy for Excellence in Engineering Education, 2013-2014
Bliss Faculty Scholar, University of Illinois at Urbana-Champaign, Urbana, IL., 2012-2016
Associate Director, University of Illinois Chicago Cancer Center, 2012-2016
Associate Professor, Department of Bioengineering,University of Illinois at Urbana-Champaign, Urbana, IL, 2011-2012
Assistant Professor, Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 2005-2011

Resident Instruction

Virtual BioImaging Laboratory - Development during 2006–2008, offered in Spring 2008, 2010, and 2011 as part of BIOE 507, Fall 2009 as part of ECE 415/BIOE 415 (Developed New Course)
BIOE 498 Senior Design - Typically one team every year since 2006
BIOE 120 Introduction to Bioengineering—1 module (Tissue Engineering) Fall 2006–Fall 2012 (Developed New Course)
BIOE 507: Advanced Bioinstrumentation +Spring 2011, Spring 2010, +Spring 2008, Offered as BIOE598 in Spring 2007 (+ University-wide List of Teachers Rated Excellent) (Developed New Course)
BIOE 201: Conservation Principles in Bioengineering +Fall 2011, +Fall 2009, +Fall 2008, +Fall 2007, +Fall 2006, Offered as BIOE 298 in Spring 2006 (+ University-wide List of Teachers Rated Excellent) (Developed New Course)
BIOE 598RB: Preparing Future Faculty +Spring 2012, +Spring 2013 (+ University-wide List of Teachers Rated Excellent) (Developed New Course)
BIOE 502 Professionalism and Ethics—1 module (Conflict of Interest) Fall 2007, Fall 2008, Fall 2010, Spring 2014 (Developed New Course)
BIOE 199: Frontiers in Cancer Research +Fall 2014, +Fall 2015 (+ University-wide List of Teachers Rated Excellent) (Developed New Course)
BIOE 298: Healthcare Innovation +Fall 2015 (+ University-wide List of Teachers Rated Excellent) Developed New Course)
BIOE 360: Transport Phenomena Spring 2015, Spring 2016
BIOE 599: Tissue Microenvironment Spring 2017, Spring 2018 (Developed New Course)
BIOE 571: Biological Measurement I (one module) Fall 2015, Spring 2017, + Spring 2018, Spring 2019 (+ University-wide List of Teachers Rated Excellent) (Developed New Course)

Research Interests

Stimulated Raman Scattering Microscopy (SRSM)
Microreactors for Continuous Fluidic Synthesis of NanoImaging Probes
3D Printing for Cell Culture Systems
Cancer pathology
Nano Scale IR Tomography
Fourier transform infrared spectroscopy
Chemical Imaging - infrared and Raman

Books Edited or Co-Edited (Original Editions)

R. Bhargava, I. W. Levin, eds. Spectrochemical Analyses Using Multichannel Infrared Detectors, Blackwell Publishing Ltd., Oxford, UK, 2005.

Chapters in Books

S. Biswas, M. J. Walsh, R. Bhargava "Fourier transform infrared (Ft-Ir) spectroscopic imaging for solid tumor histopathology" Optical Spectroscopy and Computational Methods in Biology and Medicine, Challenges and Advances in Computational Chemistry and Physics, M. Baranska, ed., Springer (2014).
K. Yeh, R. K. Reddy, R. Bhargava "Fourier transform infrared spectroscopic imaging" Emerging Imaging Technologies in Medicine, M. A. Anastasio, P. La Riviere, eds., Taylor Francis/CRC Press, Boca Raton, FL (2012).
M. J. Walsh, R. Bhargava "Infrared spectroscopic imaging: An integrative approach to pathology" Nanobiophotonics, G. Popescu, ed., McGraw-Hill, New York, NY (2010).
F. N. Pounder, R. Bhargava "Toward automated breast histopathology using mid-IR spectroscopic imaging" Vibrational Spectroscopic Imaging for Biomedical Applications, G. Srinivasan, ed., McGraw-Hill, New York, NY (2010).
R. K. Reddy, R. Bhargava "Chemometric methods for biomedical vibrational spectroscopy and imaging" Emerging Raman Applications and Techniques in Biomedical and Pharmaceutical Fields, M.D. Morris, P. Matousek, eds., Springer-Verlag, Berlin, Germany (2010).
A. K. Kodali, R. Bhargava "Nanostructured probes to enhance optical and vibrational spectroscopic imaging for biomedical applications" The Oxford Handbook of Nanoscience and Technology: Vol. III: Applications, Y. Y. Fu, A. Narlikar, eds., Oxford University Press, Oxford, UK (2010).
R. Bhargava, I. W. Levin, "Infrared spectroscopic imaging protocols for high-throughput histopathology" Vibrational Spectroscopy for Medical Diagnosis, M. Diem, P. R. Griffiths, J. Chalmers, eds., John Wiley and Sons, Chichester, UK (2008).
R. Bhargava, M. D. Schaeberle, I. W. Levin "Raman and mid-infrared microspectroscopic imaging" Vibrational Spectroscopy of Biological and Polymeric Materials, V. G. Gregariou, M. Braiman, eds., CRC Press, Boca Raton, FL (2005).
R. Bhargava, I. W. Levin "Mid-infrared spectroscopic imaging with multichannel detectors" Spectrochemical Analyses Using Infrared Multichannel Detectors, R. Bhargava, I. W. Levin, eds., Blackwell Publishing (2005).
R. Bhargava, I. W. Levin "Recent developments in Fourier transform infrared (FTIR) microspectroscopic methods for biomedical analyses: From single-point detection to two-dimensional imaging" Biomedical Photonics Handbook, T. Vo-Dinh, ed., CRC Press, Boca Raton, FL (2002).

Selected Articles in Journals

K. Falahkheirkhah, S. Tiwari, K. Yeh, S. Gupta, L. Herrera-Hernandez, M.R. McCarthy, R.E. Jimenez, J.C. Cheville, R. Bhargava “Deepfake histological images for enhancing digital pathology” Lab. Invest. In press (2022)
S. Tiwari, K. Falahkheirkhah, G. Cheng, R. Bhargava “Colon Cancer Grading Using Infrared Spectroscopic Imaging-Based Deep Learning”, Appl. Spectrosc. 76 (4), 475-484 (2022).
S. Kenkel, M. Gryka, L. Chen, M.P. Confer, A. Rao, S. Robinson, K.V. Prasanth, R. Bhargava “Chemical imaging of cellular ultrastructure with null-deflection infrared spectroscopy” Proc. Natl. Acad. Sci. In press (2022)
S. Mittal, J. Kim, R. Bhargava “Statistical Considerations and Tools to Improve Histopathologic Protocols with Spectroscopic Imaging”, Appl. Spectrosc., 76 (4), 428-438 (2022).
L. Lux, Y. Phal, P-H. Hsieh, R. Bhargava “On the Limit of Detection in Infrared Spectroscopic Imaging Appl. Spectrosc. 76 (1), 105-117 (2022).
Y. Phal, L. Pfister, P.S. Carney, R. Bhargava "Resolution Limit in Infrared Chemical Imaging", J. Phys. Chem. C, 126 (23), 9777–9783 (2022)
Y. Phal, K. Yeh, R. Bhargava “Concurrent Vibrational Circular Dichroism Measurements with Infrared Spectroscopic Imaging” Anal. Chem. 93, 3, 1294–1303 (2021). DOI: 10.1021/acs.analchem.0c00323
Y. Phal, K. Yeh, R. Bhargava “Design Considerations for Discrete Frequency Infrared Microscopy Systems” Appl. Spectrosc. 75 (9), 1067-1092 (2021)
Migliaccio, C.T., Hamilton, R.F., Shaw, P.K. et al. “Respiratory and systemic impacts following MWCNT inhalation in B6C3F1/N mice” Part Fibre Toxicol 18, 16 (2021). DOI: 10.1186/s12989-021-00408-z
K. Falahkheirkhah, T. Guo, M. Hwang, P. Tamboli, C.G. Wood, J.A. Karam, K. Sircar, R. Bhargava “A generative adversarial approach to facilitate archival-quality histopathologic diagnoses from frozen tissue sections” Lab. Invest. (2021). DOI: 10.1038/s41374-021-00718-y
C.T. Migliaccio, R.F. Hamilton, P.K. Shaw, J.F. Rhoderick, S. Deb, R. Bhargava, J.R. Harkema, A. Holian "Respiratory and systematic impacts following MWCNT inhalation in B6C3F1/N mice" Particle and fibre toxicology 18(1), 1-21 (2021). DOI: https://doi.org/10.1186/s12989-021-00408-z
K. Falahkheirkhah, K. Yeh, S. Mittal, L. Pfister, R. Bhargava "Deep learning-based protocols to enhance infrared imaging systems" Chemometrics and Intelligent Laboratory Systems (2021). DOI: https://doi.org/10.1016/j.chemolab.2021.104390
E. Zimmerman, S. Mukherjee, K. Falahkheirkhah, M. Gryka, A. Kajdacsy-Balla, W. Hasa, G. Giraud, F. Tibayan, J. Raman, R. Bhargava "Detection and Quantification of Myocardial Fibrosis Using Stain-Free Spectroscopic Imaging" Archives of Pathology & Laboratory Medicine (2021). DOI: https://doi.org/10.5858/arpa.2020-0635-OA
Y. Phal, K. Yeh, R. Bhargava "EXPRESS: Design Considerations for Discrete Frequency Infrared Microscopy Systems" Applied Spectroscopy (2021). DOI: https://doi.org/10.1177%2F00037028211013372
Y. Phal, K. Yeh, R. Bhargava "Discrete Frequency Infrared Vibrational Circular Dichroism Spectroscopy & Imaging" Novel Techniques in Microscopy (2021)
R. Bhargava, K. Falahkheirkhah "Enhancing hyperspectral imaging" Nature Machine Intelligence, 3(4), 279-280 (2021). DOI: https://doi.org/10.1038/s42256-021-00336-9
R. Bhargava "Pushing the limits of spatial and temporal capability of infrared spectroscopic imaging: a theory-based approach" Adv. Chem. Microscopy for Life Science and Translational Medicine, 11656 (2021)
Y. Phal, K. Yeh, R. Bhargava "Mid-IR laser-based polarimetric imaging for polymeric and biological applications" Adv. Chem. Microscopy for Life Science and Translational Medicine, 11656 (2021)
S. Mittal, R. Bhargava "Statistical considerations in spectral histopathology and performance evaluation" Adv. Chem. Microscopy for Life Science and Translational Medicine, 11656 (2021). DOI: https://doi.org/10.1117/12.2577337
G. Azarfar, K. Falahkheirkhah, M. Gopu, J. Pfister, S.V. Brodsky, T. Nadasdy, G. Cheng, A.A. Satoskar, R. Bhargava "Infrared chemical imaging for histologic interpretation of renal tissue" Adv. Chem. Microscopy for Life Science and Translational Medicine, 11656 (2021). DOI: https://doi.org/10.1117/12.2577337
M.A.B. Emon, S. Knoll, U. Doha, L. Ladehoff, L. Lalonde, D. Baietto, M. Sivaguru, R. Bhargava, M. Taher A. Saif " Dose-independent threshold illumination for non-invasive time-lapse fluorescence imaging of live cells" Extreme Mechanics Letters, 101249 (2021). DOI: https://doi.org/10.1016/j.eml.2021.101249
S. Mittal, T.P. Wrobel, M. Walsh, A. Kajdacsy-Balla, R. Bhargava "Breast cancer histopathology using infrared spectroscopic imaging: The impact of instrumental configuerations" Clinical Spectroscopy, 100006 (2021). DOI: https://doi.org/10.1016/j.clispe.2021.100006
S. Tiwari, A. Kajdacsy-Balla, J. Whiteley, G. Cheng, S.M. Hewitt, R. Bhargava "INFORM: INFrared-based ORganizational Measurements of tumor and its microenvironment to predict patient survival" Science Advances 7(6), eabb8292 (2021). DOI: https://doi.org/10.1126/sciadv.abb8292
Y. Phal, K. Yeh, R. Bhargava "Concurrent Vibrational Circular Dichroism Measurements with Infrared Spectroscopic Imaging" Analytical Chemistry (2020). DOI: https://dx.doi.org/10.1021/acs.analchem.0c00323
B.P. Smith, L.S. Auvil, M. Welge, C.B. Bushell, R. Bhargava, N. Elango... "Identification of early liver toxicity gene biomarkers using comparative supervised machine learning" Scientific Reports 10(1), 1-27 (2020). DOI: https://doi.org/10.1038/s41598-020-76129-8
L. Pfister, R. Bhargava, Y. Bresler, P.S. Carney "Composition-aware spectroscopic tomography" Inverse Problems 36(11), 115010 (2020)
L. Chen, S.M. Kenkel, P.H. Hsieh, M.C. Gryka, R. Bhargava "Freeform Three-Dimensionally Printed Microchannels via Surface-Initiated Photopolymerization Combined with Sacrificial Molding" ACS Applied Materials & Interfaces 12(44), 50105-50112 (2020). DOI: https://doi.org/10.1021/acsami.0c12158
M. Schnell, S. Gupta, T.P. Wrobel, M.G. Drage, R. Bhargava, P. S. Carney, "High-resolution label-free imaging of tissue morphology with confocal phase microscopy" Optica In press (2020) DOI: also on arXiv:2003.04141
S. Kenkel, S. Mittal, R. Bhargava "Closed-Loop Atomic Force Microscopy-Infrared (AFM-IR) Spectroscopic Imaging for nanoscale molecular characterization" Nat. Comm. 11, 3225 (2020). DOI: https://doi.org/10.1038/s41467-020-17043-5
S. Tiwari, T. Triluzi, S.E. Holton, V. Regondi, B. Paolini, E. Tagliabue, R. Bhargava "Infrared Spectroscopic Imaging Visualizes a Prognostic Extracellular Matrix-Related Signature in Breast Cancer" Sci. Rep. 10, 5442 (2020). DOI: 10.1038/s41598-020-62403-2
N.B. Schorr, M.J. Counihan, R. Bhargava, J. RodrÃguez-LÃ³pez "Impact of Plasmonic Photothermal Effects on the Reactivity of Graphene Electrodes Visualized using Scanning Electrochemical Microscopy" Anal. Chem. 92(5), 3666-3673 (2020)
C. Richard, R. McGee, A. Goenka, P. Mukherjee, R. Bhargava "On-demand Milifluidic Synthesis of Quantum Dots in Digital Droplet Reactors" Ind. Engg. Chem. Res. 59(9), 3730-3735 (2020) doi: 10.1021/acs.iecr.9b04230 (2020)
M. Schnell, S. Mittal, K. Falahkheirkhah, A. Mittal, K. Yeh, S. Kenkel, A. Kadjacsy-Balla, P.S. Carney, R. Bhargava "Infrared-Optical Hybrid (IR-OH) Microscopy for Label-Free Histopathology" Frontiers in Optics (2020). DOI: https://doi.org/10.1364/FIO.2020.FW7E.5
A. Tariq , Q. Hao , Q. Sun, D.K. Singh, M. Jadaliha, Y. Zhang, N. Chetlangia, J. Ma, S.E. Holton, R. Bhargava, A. Lal, S.G. Prasanth, K.V. Prasanth "LncRNA-mediated regulation of SOX9 expression in basal subtype breast cancer cells" RNA 26(2), 175-185 (2020) doi: 10.1261/rna.073254.119. Epub 2019 Nov 5. PMID: 31690584; PMCID: PMC6961546
M. Jadaliha, O. Gholamalamdari, W. Tang, Y. Zhang, A. Petracovici, Q. Hao, A. Tariq, T.G. Kim, S.E. Holton, D.K. Singh, X.L. Li, S. Freier, S. Ambs, R. Bhargava, A. Lal, S.G. Prasanth, J. Ma, K.V. Prasanth "A natural antisense lncRNA controls breast cancer progression by promoting tumor suppressor gene mRNA stability" PLoS Genet. 29;14(11):e1007802. doi: 10.1371/journal.pgen.1007802 (2019)
S. Mittal, C. Stoean, A. Kajdacsy-Balla and R. Bhargava "Digital Assessment of Stained Breast Tissue Images for Comprehensive Tumor and Microenvironmental Analysis" Frontiers Bioengineering Biotech. In press (Invited), 7, art. 246 (2019) doi: 10.3389/fbioe.2019.00246
H. Jang, A.S. Pawate, R. Bhargava, P.J. A. Kenis "Polymeric microfluidic continuous flow mixer combined with hyperspectral FT-IR imaging for studying rapid biomolecular events" Lab. Chip 19, 2598-2609 (2019). DOI: 10.1039/C9LC00182D
I.L. Rasskazov, R. Singh, P.S. Carney, R. Bhargava "Extended Multiplicative Signal Correction for Infrared Microspectroscopy of Fibers" Appl. Spectrosc. 73, 859-869 (2019) DOI: 10.1177/0003702819844528
P. Mukherjee, A. Ghosh, M. Lamborn, M. Monwar, P. DesLauriers, R. Bhargava "Relating Strain Hardening Moduli in High Density Polyethylene Copolymers to Anisotropic Molecular Deformation Using Infrared Spectroscopic Imaging" Polymer 172, 398-403 (2019)
S. Ghosh, S. Mittal, R. Bhargava, C. Bajaj "A Fully Automated, Faster Noise Reduction Approach to Increasing the Analytical Capability of Chemical Imaging for Digital Histopathology" PLoS One, 14 (4), e0205219 (2019)
I. Srivastava, D. Sar, P. Mukherjee, A. Schwartz-Duval, Z. Huang, C. Jaramillo, A. Civantos, I. Tripathi, J.P. Allain, R. Bhargava, D. Pan "Enzyme-catalysed biodegradation of carbon dots follows sequential oxidation in a time dependent manner" Nanoscale 11, 8226-8236 (2019) DOI:10.1039/C9NR00194H
S. Mittal, R. Bhargava "A comparison of mid-infrared spectral regions on accuracy of tissue classification" Analyst 144, 2635-2642 (2019). DOI:10.1039/C8AN01782D
M.K. Gelber, G. Hurst, R. Bhargava "Freeform Assembly Planning" IEEE Tran. Auto. Sci. Engg. 16 (3) 1315 â€“ 1329 (2019), arXiv preprint arXiv:1801.00527, DOI: 10.1109/TASE.2018.2878670
K. Yeh, D. Lee, R. Bhargava "Multicolor Discrete Frequency Infrared Spectroscopic Imaging" Anal. Chem 91 (3), 2177-2185 (2019)
M.C. Gryka, T.J. Comi, R.A. Forsyth, P.M. Hadley, S. Deb, R. Bhargava "Controlled dissolution of freeform 3D printed carbohydrate glass scaffolds in hydrogels using a hydrophobic spray coating" Add. Manufact. 26, 193-201 (2019)
S. Berisha, I. Gurcan, J. Jahanipour, M. Lotfollahi, R. Bhargava, H.V. Nguyen, D. Mayerich "Deep learning for FTIR histology: leveraging spatial and spectral features with convolutional neural networks" Analyst 144, 1642-1653 (2019) DOI: 10.1039/C8AN01495G
R. Singh, T.P. Wrobel, P. Mukherjee , M. Gryka, M. Kole, S, Harrison, R. Bhargava "Bulk Protein and Oil Prediction in Soybeans Using Transmission Raman Spectroscopy: A Comparison of Approaches to Optimize Accuracy" Appl. Spectrosc. 73, 687-697 (2019)
M. Jadaliha, O. Gholamalamdari, W. Tang, Y. Zhang, A. Petracovici, Q. Hao, A.Tariq, T. Kim, S.E. Holton, D.K. Singh, X. Li, S. Freier, S. Ambs, R. Bhargava, A. Lal, S.G. Prasanth, J. Ma, K.V. Prasanth â€œA natural antisense lncRNA controls breast cancer progression by promoting tumor suppressor gene mRNA stability" PLoS Genetics 14 (11), e1007802 (2018)
I.L. Rasskazov, L. Wang, C.J. Murphy, R. Bhargava, P. S. Carney "Plasmon-Enhanced Upconversion: Engineering Enhancement and Quenching at Nano and Macro Scales" Opt. Mat. Exp. 8 (12), 3787-3804 (2018)
A. Holian, R. Hamilton, Z. Wu, S. Deb, K.L. Trout, Z. Wang, R. Bhargava, S. Mitra "Lung Deposition Patterns of MWCNT Vary With Degree of Carboxylation", Nanotox. 13, 143-59 (2018)
E.K. Neumann, T.J. Comi, N. Spegazzini, J.W. Mitchell, S.S. Rubakhin, M.U. Gillette, R. Bhargava, J.V. Sweedler "Multimodal Chemical Imaging of the Brain by Combined Matrix-assisted Laser Desorption/ionization (MALDI)-Fourier trans-form ion cyclotron resonance-(FT-ICR) mass spectrometry and infrared spectroscopic imaging" Anal. Chem. 90, 11572-11580 (2018)
S. Pahlow, K. Weber, J Popp, B.R. Wood, K. Kochan, A.Ruther, D. Perez-Guaita, P. Heraud, N. Stone, A. Dudgeon, B. Gardner, R.K. Reddy, D. Mayerich, R. Bhargava â€œApplication of Vibrational Spectroscopy and Imaging to Point-of-Care Medicine: A Review" Appl. Spectrosc. 72, 52-84 (2018). Invited article. SAS 60th anniversary
S. Kenkel, A. Mittal, S. Mittal, R. Bhargava "Probe-sample interaction-independent Atomic Force Microscopy-Infrared (AFM-IR) spectroscopy: towards robust nanoscale compositional mapping" Anal. Chem. 90, 8845-8855 (2018)
S. Mittal, K. Yeh, L.S. Leslie, S. Kenkel, A. Kadjacsy-Balla, R. Bhargava "Simultaneous cancer and tumor microenvironment subtyping using confocal infrared microscopy for all-digital molecular histopathology" Proc. Natl. Acad. Sci., 115, E5651-E5660 (2018). DOI: 10.1073/pnas.1719551115
P. Mukherjee, A. Ghosh, N. Spegazzini, M. Manwar, M. Lamborn, P.J. DesLauriers, R. Bhargava "Relating post-yield mechanical behavior in polyethylenes to spatially varying molecular deformation using infrared spectroscopic imaging: homopolymers" Macromolecules 51, 3836-3844 (2018) DOI: 10.1021/acs.macromol.8b00363
M.K. Gelber, G. Hurst, T.J. Comi, R. Bhargava "Modeling, Design and Process Characterization for 3D Printing of Carbohydrate Glass" Add. Manufact., 22, 38-50 (2018) DOI: 10.1016/j.addma.2018.04.026
K. Chrabaszcz, K. Kochan, A. Fedorowicze, A. Jasztala, E. Buczeka, L.S. Leslie, R. Bhargava, K. Malek, S. Chlopicki, K.M. Marzec "FT-IR- and Raman-based biochemical profiling of the early stage of the pulmonary metastasis in breast cancer in mice" Analyst, 143, 2042-2050 (2018). DOI: 10.1039/C7AN01883E
T.P. Wrobel, R. Bhargava “Infrared spectroscopic imaging advances as an analytical technology for biomedical sciences" Anal. Chem. 90, 3, 1444-1463 (2018) DOI: 10.1021/acs.analchem.7b05330
R. Mankar, M.J. Walsh, R. Bhargava, S. Prasad, D. Mayerich "Selecting optimal features from Fourier transform infrared spectroscopy for discrete-frequency imaging" Analyst In press (2018)
A. Ganguli, A. Ornob, N. Spegazzini, Y. Liu, G. Damhorst, T. Ghonge, B. Thornton, C. J. Konopka, W. Dobrucki, S. E. Clare, R. Bhargava, A. M. Smith, F. Kosari, R. Bashir "Pixelated spatial gene expression analysis from tissue" Nat. Comm. 9, Art. number: 202 (2018)
A.M. Andrews, R. Bhargava, R. Kennedy, L. Li, J.V. Sweedler "The Chemistry of Thought: The Role of the Measurement Sciences in Brain Research" Anal. Chem. 89, 4757âˆ’4757 (2017)
A. Ghosh, P. Mukherjee, S. Deb, R. Bhargava â€œMapping Solvation Environments in Porous Metal Organic Frameworks with Infrared Chemical Imaging" J. Phys. Chem. Lett. 8, 5325-5330 (2017). DOI: 10.1021/acs.jpclett.7b02104
I.L. Rasskazov, N. Spegazzini, P. S. Carney, R. Bhargava "Dielectric Sphere Clusters as a Model to Understand Infrared Spectroscopic Imaging Data Recorded from Complex Samples" Anal. Chem. 89, 10813-10818 (2017). DOI: 10.1021/acs.analchem.7b02168
D.K. Singh, O. Gholamalamdari, X.L. Li, A. Chakraborty, Y. Zhang, S. Guang, S. Hashemikhabir, S. Tiwari, A. Thomas, V. Macias, A. Kadjacsy-Balla, R. Bhargava, S.C. Janga, J. Ma, S.G. Prasanth, A. Lal, K.V. Prasanth "PSIP1 promotes tumorigenicity in breast cancer cells by regulating the expression of genes controlling cell cycle progression" Carcinogenesis 38(10), 966-975 (2017) DOI: 10.1093/carcin/bgx062
M.K. Gelber, M.R. Kole, N. Kim, N. Aluru, R. Bhargava "Quantitative Chemical Imaging Within Non-Planar Microfluidicsâ€ Anal. Chem. 89, 1716-1723 (2017) DOI: 10.1021/acs.analchem.6b03943
T.P. Wrobel, P. Mukherjee, R. Bhargava "Rapid visualization of macromolecular orientation by discrete mid-infrared spectroscopic imaging" Analyst 142, 75-79 (2017) DOI: 10.1039/c6an01086e.
S. Berisha, T. van Dijk, R. Bhargava, P.S. Carney, D. Mayerich "BIM-Sim: Interactive Simulation of Broadband Imaging Using Mie Theory" Fron. Phys.: Opt. Phot. 5, 5 (2017) DOI: 10.3389/fphy.2017.00005
S. Kang, S.E. Lehman, M.V. Schulmerich, A. Le, T. Lee, S.K. Gray, R. Bhargava, R.G. Nuzzo "Refractive index sensing and surface-enhanced Raman spectroscopy using silver-gold layered bimetallic plasmonic crystals" Beilstein J Nanotechnol. 8, 2492-2503 (2017). doi: 10.3762/bjnano.8.249
T.Q. Doan, L.S. Leslie, S.Y. Kim, R. Bhargava, S.R. White, N. Sottos "Characterization of core-shell microstructure and self-healing performance of electrospun fiber coatings" Polymer 107, 263-272 (2016)
S. Tiwari, C. Hoke, V. Reddy, J. Raman, R. Bhargava "Translating infrared spectroscopic imaging for rapid analysis of biopsy samples using a discrete frequency approach" Anal. Chem. 88, 10183-10190 (2016) DOI: 10.1021/acs.analchem.6b02754
F. Ostadhossein, S.K. Misra, P. Mukherjee, A. Ostadhossein, E. Daza, S. Tiwari, S. Mittal, M.C. Gryka, R. Bhargava, D. Pan "Breast Cancer Therapy: Defined host-guest chemistry on Nanocarbon for sustained inhibition of cancer" Small 12, 5782-5782 (2016) DOI: 10.1002/smll.201601161
B.M. DeVetter, S. Kenkel, S. Mittal, R. Bhargava, T. Wrobel "Characterization of structure of low-e substrates and consequences for IR transflection measurements" Vib. Spectrosc. 91, 119-127 (2016) DOI: 10.1016/j.vibspec.2016.09.001
P. Mukherjee, S.J. Lim, T.P. Wrobel, R. Bhargava,* A.M. Smith* "Measuring and predicting the internal structure of semiconductor nanocrystals through Raman spectroscopy" J. Am. Chem. Soc. 138, 10887-10896 (2016) [*joint corresponding authors] DOI: 10.1021/jacs.6b03907
S.K. Misra, P. Mukherjee, H-H. Chang, S. Tiwari, M. Gryka, R. Bhargava, D. Pan "Multi-functionality Redefined with Colloidal Carotene Carbon Nanoparticles for Synchronized Chemical Imaging, Enriched Cellular Uptake and Therapy" Sci. Rep. 6, Article 29299 (2016)
T.P. Wrobel, M. Kole, R. Bhargava "Emerging trends and opportunities in discrete frequency infrared and Raman spectroscopic imaging" Spectroscopy 31, 28-45 (2016) [Invited]
J. T. Kwak, S. M. Hewitt, A. Kajdacsy-Balla, S. Sinha, R. Bhargava "Automated prostate tissue referencing for cancer detection and diagnosis" BMC Bioinformatics 17, 227 (2016) DOI 10.1186/s12859-016-1086-6
F.N. Keith, R.K. Reddy, R. Bhargava "Development of a practical spatial-spectral analysis protocol for breast histopathology using Fourier transform infrared spectroscopic imaging" Faraday Discuss. 187, 43-68 (2016) DOI: 10.1039/c5fd00199d [Invited]
S.E. Dubowsky, B. Deutsch, R. Bhargava, B. McCall "Infrared Emission Spectroscopy of Atmospheric-Pressure Ball Plasmoids" J. Mol. Spectrosc. 322, 1-8 (2016)
S.K. Misra, P. Mukherjee, A. Ohoka, A.S. Schwartz-Duval, S. Tiwari, R. Bhargava, D. Pan "Vibrational spectroscopy and imaging for concurrent cellular trafficking of co-localized Doxorubicin and deuterated phospholipid vesicles" Nanoscale 8, 2826-2831 (2016)
R. Bhargava, A. Madabhushi "Emerging themes in image informatics and molecular analysis for digital pathology" Annu. Rev. Biomed. Engr. 18, 387-412 (2016) [Invited]
S. Tiwari, R. Bhargava "Extracting Knowledge from Chemical Imaging Data Using Computational Algorithms for Digital Cancer Diagnosis" Yale J. Biol. Med. 88, 131-143 (2015)
P. Mukherjee, S. K. Misra, M. Gryka, H. Chang, S. Tiwari, W. L. Wilson, J. Scott, R. Bhargava, D. Pan "Tunable luminescent carbon nanospheres with well-defined nanoscale chemistry for synchronized imaging and therapy" Small 36, 4691-4703 (2015) DOI: 10.1002/smll.201500728
L. S. Leslie, T. P. Wrobel, D. Mayerich, S. Bindra, R. Emmadi, R. Bhargava "High definition infrared spectroscopic imaging for lymph node histopathology" PLoS One 10:e0127238 (2015) DOI: doi: 10.1371/journal.pone.0127238
B. Deutsch, R. K. Reddy, D. Mayerich, R. Bhargava, P. S. Carney "Compositional prior information in computed infrared spectroscopic imaging" J. Opt. Soc. Am. A 32, 1126-1131 (2015)
J. Han, W. Qiu, S. Tiwari, R. Bhargava, W. Gao, B. Xing "Consumer-grade polyurethane foam functions as a large and selective absorption sink for bisphenol A in aqueous media" J. Mater. Chem. A 3, 8870-8881 (2015)
S. Tiwari, V.B. Reddy, R. Bhargava, J. Raman "Computational Chemical Imaging for Cardiovascular Pathology: Chemical Microscopic Imaging Accurately Determines Cardiac Transplant Rejection" PLoS One 10, e0125183 (2015) DOI: 10.1371/journal.pone.0125183
B. M. DeVetter, P. Mukherjee, C. J. Murphy, R. Bhargava "Measuring binding kinetics of aromatic thiolated molecules with nanoparticles via surface-enhanced Raman spectroscopy" Nanoscale 7, 8766-8775 (2015) DOI: 10.1039/C5NR01006C
M. K. Gelber, R. Bhargava "Monolithic multilayer microfluidics via sacrificial molding of 3D-printed isomalt" Lab Chip 15, 1736-1741 (2015) DOI: 10.1039/c4lc01392a.
B. Chng, R. Bhargava, P. S. Carney "Enhancement and extinction effects in surface-enhanced stimulated Raman spectroscopy" Phys. Chem. Chem. Phys. 17, 21348-21355 (2015) DOI: 10.1039/c4cp05089d
J. T. Kwak, A. Kajdacsy-Balla, V. Macias, M. J. Walsh, S. Sinha, R. Bhargava "Improving prediction of prostate cancer recurrence using chemical imaging" Sci. Rep. 5, 8758 (2015)
D. Mayerich, M.J. Walsh, A. Kajdacsy-Balla, P.S. Ray, S. M. Hewitt, R. Bhargava "Stain-less staining for computed molecular histopathology" Technology 3, 27-31 (2015)
K. Yeh, S. Kenkel, J.-N. Liu, R. Bhargava "Fast infrared chemical imaging with a quantum cascade laser" Anal. Chem. 87, 485-493 (2015)
T. van Dijk, D. Mayerich, P.S. Carney, R. Bhargava "Understanding the optics-sample interaction in infrared spectroscopic imaging"Â AnalystÂ 139, 4031-4036 (2015).
B. M. DeVetter, S. T. Sivapalan, D. D. Patel, M. V. Schulmerich, C. J. Murphy, R. Bhargava "Observation of molecular diffusion in polyelectrolyte-wrapped SERS nanoprobes" Langmuir 30, 8931-8937 (2014)
J.-N. Liu, M. V. Schulmerich, R. Bhargava, B. T. Cunningham "Sculpting narrowband Fano resonances inherent in the large-area mid-infrared photonic crystal microresonators for spectroscopic imaging" Opt. Express 22, 18142-18158 (2014)
A. Rosenberg, J. Surya, R. Liu, W. Streyer, S. Law, L. S. Leslie, R. Bhargava, D. Wasserman "Flat mid-infrared composite plasmonic materials using lateral doping-patterned semiconductors" J. Optics 16, 094012 (2014)
M. J. Baker, J. Trevisan, P. Bassan, R. Bhargava, H. J. Butler, K. M. Dorling, P. R. Fielden, S. W. Fogarty, N. J. Fullwood, K. A. Heys, C. Hughes, P. Lasch, P. L. Martin-Hirsch, B. Obinaju, G. D. Sockalingum, J. Sul-Suso, R. J. Strong, M. .J. Walsh, B. R. Wood, P. Gardner, F. L. Martin "Using Fourier transform IR spectroscopy to analyze biological materials" Nat. Protocols 9, 1771-1791 (2014) doi:10.1038/nprot.2014.110.
S. E. Holton, A. Bergamaschi, B. S. Katzenellenbogen, R. Bhargava "Integration of molecular profiling and chemical imaging to elucidate fibroblast-microenvironment impact on cancer cell phenotype and endocrine resistance in breast cancer" PLOS ONE 9(5), e96878 (2014)
D. Mayerich, T. van Dijk, M. J. Walsh, M. V. Schulmerich, P. S. Carney, R. Bhargava "On the importance of image formation optics in the design of infrared spectroscopic imaging systems" Analyst 139, 4031-4036 (2014)
B. M. DeVetter, R. Bhargava, C. J. Murphy "Computational study of the surface-enhanced Raman scattering from silica-coated silver nanowires" Photochem. Photobiol. 90, 415-418 (2014)
B. Kwon, M. Seong, J.-N. Liu, M. R. Rosenberger, M. V. Schulmerich, R. Bhargava, B. T. Cunningham, W. P. King "Large infrared absorptance of bimaterial microcantilevers based on silicon high contrast gratings" J. Appl. Phys. 114, 153511 (2013) doi:10.1063/1.4825313.
M. V. Schulmerich, M. K. Gelber, H. M. Azam, S. K. Harrison, J. McKinney, D. Thompson, B. Owen, L. S. Kull, R. Bhargava "Amino acid quantification in bulk soybeans by transmission Raman spectroscopy" Anal. Chem. 85, 11376-11381 (2013)
B. Kwon, J. Jiang, M. V. Schulmerich, Z. Xu, R. Bhargava, G. L. Liu, W. P. King "Bimaterial microcantilevers with black silicon nanocone arrays" Sens. Actuators, A 199, 143-148 (2013)
T. van Dijk, D. Mayerich, R. Bhargava, P. S. Carney "Rapid spectral-domain localization" Opt. Express 21, 12822-12830 (2013)
S. T. Sivapalan, B. M. DeVetter, T. K. Yang, M. V. Schulmerich, R. Bhargava, C. J. Murphy "Surface-enhanced Raman spectroscopy of polyelectrolyte-wrapped gold nanoparticles in colloidal suspension" J. Phys. Chem. C 117, 10677-10682 (2013)
D. Mayerich, M. J. Walsh, M. V. Schulmerich, R. Bhargava "Real-time interactive data mining for chemical imaging information: Application to automated histopathology" BMC Bioinformatics 14, 156 (2013) [designated highly accessed]
T. van Dijk, D. Mayerich, P. S. Carney, R. Bhargava "Recovery of absorption spectra from Fourier transform infrared (FT-IR) microspectroscopic measurements of intact spheres" Appl. Spectrosc. 67, 546-552 (2013)
T. van Dijk, S. T. Sivapalan, B. M. DeVetter, M. V. Schulmerich, T. K. Yang, C. J. Murphy, R. Bhargava, P. S. Carney "Competition between extinction and enhancement in surface-enhanced Raman spectroscopy" J. Phys. Chem. Lett. 4, 1193-1196 (2013)
A. B. Shah, S. T. Sivapalan, B. M. DeVetter, T. K. Yang, J. Wen, R. Bhargava, C. J. Murphy, J.-M. Zuo "High-index facets in gold nanocrystals elucidated by coherent electron diffraction" Nano Lett. 13, 1840-1846 (2013)
Y. Liu, C. Zhang, D. Hu, M. S. Kuhlenschmidt, T. B. Kuhlenschmidt, S. E. Mylon, R. Kong, R. Bhargava, T. H. Nguyen "Role of collector alternating charged patches on transport of Cryptosporidium parvum oocyst in a patchwise charged heterogeneous micromodel" Environ. Sci. Technol., 47, 2670-2678 (2013) doi:10.1021/es304075j.
A. K. Kodali, M. Schulmerich, R. Palekar, R. Bhargava "Sculpting the analytical volume in and around nanoparticle sensors using a multilayer geometry" Anal. Chem. 85, 3842-3848 (2013)
S. T. Sivapalan, B. M. DeVetter, T. K. Yang, T. van Dijk, M. V. Schulmerich, P. S. Carney, R. Bhargava, C. J. Murphy "Off-resonance SERS from gold nanorod suspensions as a function of aspect ratio: Not what we thought" ACS Nano 7, 2099-2105 (2013)
A. Abouimrane, S. A. Odom, H. Tavassol, M. V. Schulmerich, H. Wu, R. Bhargava, A .A. Gewirth, J. S. Moore, K. Amine "3-Hexylthiophene as a stabilizing additive for high voltage cathodes in lithium-ion batteries" J. Electrochem. Soc. 160, A268-A271 (2013)
B. Kwon, M. V. Schulmerich, R. Bhargava, W. P. King "Infrared emission from heated microcantilevers" Nanosc. Microsc. Thermophys. Eng. 17, 141-153 (2013)
J. G. Moreno-Torres, X. LlorÃ , D. E. Goldberg, R. Bhargava "Repairing fractures between data using genetic programming-based feature extraction: A case study in cancer diagnosis" Info. Sci. 222, 805-823 (2013)
R. K. Reddy, M. J. Walsh, M. V. Schulmerich, P. S. Carney, R. Bhargava "High-definition infrared spectroscopic imaging" Appl. Spectrosc. 67, 93-105 (2013)
M. R. Kole, R. K. Reddy, M. V. Schulmerich, M. K. Gelber, R. Bhargava "Discrete frequency infrared microspectroscopy and imaging with a tunable quantum cascade laser" Anal. Chem. 84, 10366-10372 (2012) doi:dx.doi.org/10.1021/ac302513f.
R. Bhargava "Infrared spectroscopic imaging: The next generation" Appl. Spectrosc. 66, 1091-1120 (2012) [Invited Focal Point Article]
M. V. Schulmerich, M. J. Walsh, M. K. Gelber, R. Kong, M. R. Kole, S. K. Harrison, J. McKinney, D. Thompson, L. S. Kull, R. Bhargava "Protein and oil composition predictions of single soybeans by transmission Raman spectroscopy" J. Agric. Food. Chem. 60, 8097-8102 (2012)
R. Ambekar, T.-Y. Lau, M. Walsh, R. Bhargava, K. C. Toussaint Jr "Quantifying collagen structure in breast biopsies using second-harmonic generation imaging" Biomed. Opt. Express 3, 2021-2035 (2012)
S. A. Odom, T. P. Tyler, M. M. Caruso, J. A. Ritchey, M. V. Schulmerich, S. J. Robinson, R. Bhargava, N. R. Sottos, S. R. White, M. C. Hersam, J. S. Moore "Autonomic restoration of electrical conductivity using polymer-stabilized carbon nanotube and graphene microcapsules" Appl. Phys. Lett. 101, 043106 (2012)
N. Lu, S. E. Mylon, R. Kong, R. Bhargava, J. L. Zilles, T. H. Nguyen "Interactions between dissolved natural organic matter and adsorbed DNA and their effect on natural transformation of Azotobacter vinelandii" Sci. Total Environ. 426, 430-435 (2012)
M. J. Walsh, R. K. Reddy, R. Bhargava "Label-free biomedical imaging with mid-infrared microspectroscopy" IEEE J. Sel. Top. Quant. 18, 1502-1513 (2012) [Invited]
M. J. Walsh, S. E. Holton, A. Kajdacsy-Balla, R. Bhargava "Attenuated total reflectance Fourier transform infrared spectroscopic imaging for comprehensive breast tissue histopathology" Vib. Spectrosc. 60, 23-28 (2012) doi:10.1016/j.vibspec.2012.01.010. doi:10.1109/JSTQE.2011.2182635.
B. Kwon, M. V. Schulmerich, L. J. Elgass, R. Kong, S. E. Holton, R. Bhargava, W. P. King "Infrared microspectroscopy combined with conventional atomic force microscopy" Ultramicroscopy 116, 56-61 (2012)
J. T. Kwak, R. K. Reddy, S. Sinha, R. Bhargava "Analysis of variance in spectroscopic imaging data from human tissues" Anal. Chem. 84, 1063-1069 (2012) doi:10.1021/ac2026496.
B. Kwon, M. Rosenberger, R. Bhargava, D. G. Cahill, W. P. King "Dynamic thermomechanical response of bimaterial microcantilevers to periodic heating by infrared radiation" Rev. Sci. Instr. 83, 015003 (2012) doi:10.1063/1.3680107.
D. M. Mahr, R. Bhargava, M. F. Insana "Three-dimensional in silico breast phantoms for multimodal image simulations" IEEE Trans. Med. Imaging 31, 689-697 (2012) doi:10.1109/TMI.2011.2175401.
J.-N. Liu, M. V. Schulmerich, R. Bhargava, B. T. Cunningham "Optimally designed narrowband mid-infrared guided-mode resonance reflectance filters for mid-infrared optical spectroscopy" Opt. Express 19, 24182-24197 (2011) doi:10.1364/OE.19.024182.
D. Chanda, K. Shigeta, T. Truong, E. Lui, A. Mihi, M. V. Schulmerich, P. V. Braun, R. Bhargava, J. A. Rogers "Coupling of plasmonic and optical cavity modes in quasi-three-dimensional plasmonic crystals" Nat. Commun. 2, 479 (2011) doi:10.1038/ncomms1487.
A. K. Kodali, R. Bhargava "Effect of non-specificity in shape, size, and dielectric properties on electromagnetic extinction and optical field enhancement from spherical nanolayered metal-dielectric particles" Theor. Chem. Acc. 130, 991-1000 (2011) [Invited] doi:10.1007/s00214-011-1038-1.
S. E. Holton, M. J. Walsh, A. Kajdacsy-Balla, R. Bhargava "Label-free characterization of cancer-activated fibroblasts in cell culture and clinical tissues using infrared spectroscopic imaging" Biophys. J. 101, 1513-1521 (2011) doi:10.1016/j.bpj.2011.07.055.
J. Yao, A.-P. Le, M. V. Schulmerich, J. Maria, T.-W. Lee, S. K. Gray, R. Bhargava, J. A. Rogers, R. G. Nuzzo "Soft embossing of nanoscale optical and plasmonic structures in glass" ACS Nano 5, 5763-5774 (2011) doi:10.1021/nn201464t.
S. E. Holton, M. J. Walsh, R. Bhargava "Subcellular localization of early biochemical transformations in cancer-activated fibroblasts using high-resolution infrared spectroscopic imaging" Analyst 136, 2953-2958 (2011) doi:10.1039/c1an15112f.
R. Kong, R. Bhargava "Characterization of porcine skin as a model for human transdermal diffusion" Analyst 136, 2359-2366 (2011) doi:10.1039/C1AN15111H.
M. J. Nasse, M. J. Walsh, E. C. Mattson, R. Reininger, A. Kajdacsy-Balla, V. Macias, R. Bhargava,* C. J. Hirschmugl* "High-resolution Fourier transform infrared chemical imaging with multiple synchrotron beams" Nat. Methods 8, 413-416 (2011) doi:10.1038/NMETH.1585 [*Joint corresponding authors]
J. T. Kwak, S. M. Hewitt, S. Sinha, R. Bhargava "Multimodal microscopy for automated histologic analysis of prostate cancer" BMC Cancer 11, 62 (2011) [Designated Highly Accessed]. * Author profile by M. Baker Nat. Methods 8, 363 (2011) * News and Views: F. L. Martin "Shining a new light into molecular workings" Nat. Methods 8, 385-387 (2011)
B. J. Davis, P. S. Carney, R. Bhargava "Theory of infrared microspectroscopy for intact fibers" Anal. Chem. 83, 525-532 (2011)
B. Kwon, C. Wang, K. Park, R. Bhargava, W. P. King "Thermomechanical sensitivity of microcantilevers in the mid-infrared spectral region" Nano Micro Thermophys. Eng. 15, 16-28 (2011)
J. G. Moreno-Torres, X. LlorÃ , D. E. Goldberg, R. Bhargava "On the homogenization of data from two laboratories using genetic programming" Lec. Notes Comp. Sci. 6471, 185-197 (2010) doi:10.1007/978-3-642-17508-4_12.
A. K. Kodali, M. V. Schulmerich, R. Palekar, X. Llora, R. Bhargava "Optimized nanospherical layered alternating metal-dielectric probes for optical sensing" Opt. Express 18, 23302-23313 (2010)
R. K. Reddy, R. Bhargava "Accurate histopathology from low signal-to-noise ratio spectroscopic imaging data" Analyst 135, 2818-2825 (2010) doi:10.1039/C0AN00350F.
A. K. Kodali, X. Llora, R. Bhargava "Optimally designed nanolayered metal-dielectric particles as probes for massively multiplexed and ultrasensitive molecular assays" Proc. Natl. Acad. Sci. 107, 13620-13625 (2010) doi:10.1073/pnas.1003926107.
A. K. Kodali, M. Schulmerich, J. Ip, G. Yen, B. T. Cunningham, R. Bhargava "Narrowband midinfrared reflectance filters using guided mode resonance" Anal. Chem. 82, 5697-5706 (2010) doi:10.1021/ac1007128.
M. V. Schulmerich, R. Reddy, A. K. Kodali, L. J. Elgass, K. Tangella, R. Bhargava "Dark field Raman microscopy" Anal. Chem. 82, 6273-6280 (2010) doi:10.1021/ac1014194.
R. Kong, R. K. Reddy, R. Bhargava "Characterization of tumor progression in engineered tissue using infrared spectroscopic imaging" Analyst 135, 1569-1578 (2010) doi:10.1039/c0an00112k.
B. J. Davis, P. S. Carney, R. Bhargava "Theory of mid-infrared absorption microspectroscopy. II. Heterogeneous samples" Anal. Chem. 82, 3487-3499 (2010) doi:10.1021/ac902068e.
B. J. Davis, P. S. Carney, R. Bhargava "Theory of mid-infrared absorption microspectroscopy. I. Homogeneous samples" Anal. Chem. 82, 3474-3486 (2010) doi:10.1021/ac902067p.
R. Bhargava, R. S. Perlman, D. C. Fernandez, I. W. Levin, E. G. Bartick "Non-invasive detection of superimposed latent fingerprints and inter-ridge trace evidence by infrared spectroscopic imaging" Anal. Bioanal. Chem. 394, 2069â€“2075 (2009)
X. LlorÃ , A. Priya, R. Bhargava "Observer-invariant histopathology using genetics-based machine learning" Nat. Computing 8, 101-120 (2009)
K. Park, J. Lee, R. Bhargava, W. P. King "Routine femtogram-level chemical analyses using vibrational spectroscopy and self-cleaning scanning probe microscopy tips" Anal. Chem. 80, 3221-3228 (2008)
R. Bhargava "Towards a practical Fourier transform infrared chemical imaging protocol for cancer histopathology" Anal. Bioanal. Chem. 389, 1155-1169 (2007)
G. Srinivasan, R. Bhargava "Fourier transform infrared spectroscopic imaging: the emerging evolution from a microscopy tool to a cancer imaging modality" Spectroscopy, 22, 40-51 (2007)
R. Bhargava, S. M. Hewitt, I. W. Levin "Reply to Unrealistic expectations for IR microspectroscopic imaging" Nat. Biotechnol. 25, 31-33 (2007)
R. Bhargava, D. C. Fernandez, S. M. Hewitt, I. W. Levin "High throughput assessment of cells and tissues: Bayesian classification of spectral metrics from infrared vibrational spectroscopic imaging data" Biochim. Biophys. Acta 1758, 830-845 (2006)
D. C. Fernandez,* R. Bhargava,* S. M. Hewitt, I. W. Levin "Infrared spectroscopic imaging for histopathologic recognition" Nat. Biotechnol. 23, 469-474 (2005) [*equal first authors]
I. W. Levin, R. Bhargava "Fourier transform infrared vibrational spectroscopic imaging: Integration of microscopy and molecular recognition" Annu. Rev. Phys. Chem. 56, 429-474 (2005)
R. Bhargava, I. W. Levin "Gram-Schmidt orthogonalization for rapid reconstructions of Fourier transform infrared spectroscopic imaging data" Appl. Spectrosc. 58, 995-1000 (2004)
R. Bhargava, I. W. Levin "Enhanced time-resolved Fourier transform infrared spectroscopic imaging for reversible dynamics" J. Phys. Chem. A 108, 3896-3901 (2004)
R. Bhargava, I. W. Levin "Probing the structure and dynamics of macromolecular composites by infrared spectroscopy and imaging" Vib. Spectrosc. 34, 13-24 (2004) [Invited Special Issue: Prominent Young Vibrational Spectroscopists]
R. Bhargava, S.-Q. Wang, J. L. Koenig "FTIR microspectroscopy of polymeric systems" Adv. Polym. Sci. 163, 137, 191 (2003)
R. Bhargava, I. W. Levin "Time-resolved Fourier transform infrared spectroscopic imaging" Appl. Spectrosc. 57, 357-366 (2003) [Accelerated Publication].
R. Bhargava, I. W. Levin "Noninvasive imaging of molecular dynamics in heterogeneous materials" Macromolecules 36, 92-96 (2003) [Featured in Anal. Chem. News Section, July 2003]
S. W. Huffman, R. Bhargava, I. W. Levin "A generalized implementation of rapid-scan Fourier transform infrared spectroscopic imaging" Appl. Spectrosc. 56, 965-969 (2002) [Accelerated Publication]
R. Bhargava, I. W. Levin "Effective time averaging of multiplexed measurements: A critical analysis" Anal. Chem. 74, 1429-1435 (2002)
I. W. Levin, R. Bhargava "Fourier transform infrared spectroscopic imaging of biological tissue" Trends Appl. Spectrosc. 3, 57-71 (2001)
R. Bhargava, D. C. Fernandez, M. D. Schaeberle, I. W. Levin "Theory and application of gain ranging to Fourier transform infrared spectroscopic imaging" Appl. Spectrosc. 55, 1580-1589 (2001)
R. Bhargava, I. W. Levin "FTIR imaging: Theory and practice" Anal. Chem. 73, 5157-5167 (2001)
T. Ribar, J. L. Koenig, R. Bhargava "FTIR imaging of polymer dissolution. 2. Solvent/nonsolvent mixtures" Macromolecules 34, 8340-8346 (2001)
R. Bhargava, M. D. Schaeberle, D. C. Fernandez, I. W. Levin "Novel route to faster Fourier transform infrared spectroscopic imaging" Appl. Spectrosc. 55, 1079-1084 (2001)
J. L. Koenig, S.-Q. Wang, R. Bhargava "FTIR images: A new technique produces images worth a thousand spectra" Anal. Chem. 73, 361A-369A (2001) [Cover Feature Article]
R. Bhargava, D. C. Fernandez, M. D. Schaeberle, I. W. Levin "Effect of focal plane array cold shield aperture size on Fourier transform infrared micro-imaging spectrometer performance" Appl. Spectrosc. 54, 1743-1750 (2000)
T. Ribar, R. Bhargava, J. L. Koenig "FT-IR imaging of polymer dissolution by solvent mixtures. 1. Solvents" Macromolecules 33, 8842-8849 (2000)
R. Bhargava, S.-Q. Wang, J. L. Koenig "Processing FT-IR imaging data for morphology visualization" Appl. Spectrosc. 54, 1690-1706 (2000)
R. Bhargava, B. G. Wall, J. L. Koenig "Comparison of the FT-IR mapping and imaging techniques applied to polymeric systems" Appl. Spectrosc. 54, 470-479 (2000)
R. Bhargava, S.-Q. Wang, J. L. Koenig "Route to higher fidelity FT-IR imaging" Appl. Spectrosc. 54, 486-495 (2000)
R. Bhargava, S.-Q. Wang, J. L. Koenig "Studying polymer-dispersed liquid-crystal formation by FTIR spectroscopy. 1. Monitoring curing reactions" Macromolecules 32, 8982â€“8988 (1999)
R. Bhargava, S.-Q. Wang, J. L. Koenig "Studying polymer-dispersed liquid-crystal formation by FTIR spectroscopy. 2. Phase separation and ordering" Macromolecules 32, 8989â€“8995 (1999)
R. Bhargava, T. Ribar, J. L. Koenig "Towards faster FT-IR imaging by reducing noise" Appl. Spectrosc. 53, 1313-1322 (1999)
R. Bhargava, S.-Q. Wang, J. L. Koenig "FTIR imaging studies of a new two-step process to produce polymer dispersed liquid crystals" Macromolecules 32, 2748, 2760 (1999)
R. Bhargava, S.-Q. Wang, J. L. Koenig "FT-IR imaging of the interface in multicomponent systems using optical effects induced by differences in refractive index" Appl. Spectrosc. 52, 323, 328 (1998) [Accelerated Publication].
U. S. Agarwal, R. Bhargava, R. A. Mashelkar "Brownian dynamics simulation of a polymer molecule in solution under elongational flow" J. Chem. Phys. 108, 1610, 1617 (1998)

Pending Articles

R. Bhargava “Digital histopathology by infrared spectroscopic imaging” Ann. Rev. Anal. Chem.
L. Chen, P. Hsieh. S.M. Kenkel, M.K. Gelber, R. Bhargava “Bi-Layered 3D Tubular Constructs with Enhanced Cellular Adhesion via Wavelength-Selective Surface-Initiated Photopolymerization” Biofab.
R. Ho, Y. Phal, K. Falahkheirkhah, K. Yeh, J. Mitchell, S.S. Rubakhin, M. Gillette, J.V. Sweedler, R. Bhargava “Multi-section structural and chemical mapping of whole rat brains by infrared spectroscopic imaging” Anal. Chem.
P. Hsieh, Y. Phal, K.V. Prasanth, R. Bhargava “Cell Phase Identification in a Three-Dimensional Engineered Tumor Model by Infrared Spectroscopic Imaging” Anal. Chem.

Patents

R Bhargava, S Kenkel “Method and apparatus for correcting responsivity variation in photothermal imaging” US Patent 11,143,672 (2021)
Y. Phal, K.L. Yeh, R. Bhargava “Vibrational circular dichroism infrared spectroscopic imaging microscope” US Patent App. 17/377,096 (2022)
M. Schnell, P.S. Carney, R. Bhargava “Infrared-optical hybrid imaging technology” US Patent App. 16/733,610 (2022)
R Bhargava, KL Yeh “Method and apparatus for multicolor discrete frequency infrared spectroscopic imaging”, US Patent appl. 17/135,010 (2022)

Service on Campus Committees

Chair, Search Committee for Director, School of Chemical Sciences, College of Liberal Arts & Sciences (2021-2022)

Teaching Honors

Outstanding Advisor, Grainger College of Engineering (2020)
Rose Award for Teaching Excellence (2012)
Teaching Academy Faculty Fellow in Engineering (2010, 2011, 2012)
Everitt Award for Teaching Excellence (2009)
Society for Applied Spectroscopy National Tour Speaker (2008)
Accenture Outstanding Faculty Award for Undergraduate Advising (2007, 2009)
Lists of Teachers Ranked as Excellent, 13 times (2006-present)
AE3 Scholar, Academy for Excellence in Engineering Education (2005)

Research Honors

Society for Applied Spectroscopy Gold Medal, SAS NY/NJ Section (2022)
Pittsburgh Spectroscopy Award (2022)
Fellow, Optica (2022)
Ellis R. Lippincott Award, The Optical Society, Coblentz Society, and Society for Applied Spectroscopy (2021)
Fellow, American Association for the Advancement of Science (2020)
Beckman Vision and Spirit Award (2017)
Agilent Thought Leader Award (2016)
Fellow, American Institute for Medical and Biological Engineering (2015)
Fellow, Society for Applied Spectroscopy (2015)
William F. Meggers Award, Society for Applied Spectroscopy (2014)
Craver Award (2013)
FACSS Innovation Award (2012)
Campus award for outstanding promotion and tenure dossier (2011)
Xerox Award for Faculty Research (2011)
Fellow, Center for Advanced Study (2010)
Bronze Medal, Human-Competitive Competition, GECCO07 (2007)
DoD Young Investigator, Prostate Cancer Research program (2007)
Faculty Fellow, National Center for Supercomputing Applications (2006)
Best Manuscript, Prominent Young Vibrational Spectroscopists' Issue of Vibrational Spectroscopy (2004)
William F. Meggers Award, Society for Applied Spectroscopy (2002)
Gordon F. Kirkbright Bursary Award, Association of British Spectroscopists (2001)
Case Alumni Award for Best Graduate Spectroscopist (2000)
Sigma Pi Epsilon Honor Society, Society of Plastic Engineers (2000)
William F. Meggers Award, Society for Applied Spectroscopy (1999)
Samuel H. Maron Award, Case Western Reserve University (1998)
The Roll of Honour for Outstanding Cumulative Contribution as an Undergraduate (1996)
The Undergraduate Research Award, IIT-D (1994)
National Science Talent Search Scholar, NCERT, India (1989-1996)